Invention Grant
- Patent Title: Confirmation test for primary nucleic acid amplification products in a continuous reaction setup and immediate analysis via immunochromatographic methods
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Application No.: US15760836Application Date: 2016-09-15
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Publication No.: US10669573B2Publication Date: 2020-06-02
- Inventor: Werner Brabetz
- Applicant: Biotype GmbH
- Applicant Address: DE Dresden
- Assignee: Biotype GmbH
- Current Assignee: Biotype GmbH
- Current Assignee Address: DE Dresden
- Agency: Jenkins, Wilson, Taylor & Hunt, P.A.
- Priority: com.zzzhc.datahub.patent.etl.us.BibliographicData$PriorityClaim@99f4735
- International Application: PCT/EP2016/071746 WO 20160915
- International Announcement: WO2017/046191 WO 20170323
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12Q1/6844 ; C12Q1/6853 ; C12Q1/6823 ; C12Q1/6851

Abstract:
The invention relates to a method for confirming an amplified nucleic acid target sequence (target sequence), preferably from human samples, during a multiplication reaction in a collective and continuous reaction batch as a one-pot process, wherein the confirmation of the target sequence amplification product is obtained by means of a hapten-pair-marked artificial template amplification product. The artificial template sequence is amplified and optionally marked by means of the 5′-cleavage products of the at least one target-sequence-specific FEN probe. The 5′-cleavage product of the at least one target-sequence-specific FEN probe is obtained only if the FEN probe hybridizes, by means of the target-sequence-specific 3′ sequence thereof, to a complementary sequence segment of the at least one target sequence. The detection of the obtained plurality of template amplification products occurs distinctly and preferably by means of immunochromatographic methods.
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