• Patent Title: Confirmation test for primary nucleic acid amplification products in a continuous reaction setup and immediate analysis via immunochromatographic methods
  • Application No.: US15760836
    Application Date: 2016-09-15
  • Publication No.: US10669573B2
    Publication Date: 2020-06-02
  • Inventor: Werner Brabetz
  • Applicant: Biotype GmbH
  • Applicant Address: DE Dresden
  • Assignee: Biotype GmbH
  • Current Assignee: Biotype GmbH
  • Current Assignee Address: DE Dresden
  • Agency: Jenkins, Wilson, Taylor & Hunt, P.A.
  • Priority: com.zzzhc.datahub.patent.etl.us.BibliographicData$PriorityClaim@99f4735
  • International Application: PCT/EP2016/071746 WO 20160915
  • International Announcement: WO2017/046191 WO 20170323
  • Main IPC: C12Q1/68
  • IPC: C12Q1/68 C12Q1/6844 C12Q1/6853 C12Q1/6823 C12Q1/6851
Confirmation test for primary nucleic acid amplification products in a continuous reaction setup and immediate analysis via immunochromatographic methods
Abstract:
The invention relates to a method for confirming an amplified nucleic acid target sequence (target sequence), preferably from human samples, during a multiplication reaction in a collective and continuous reaction batch as a one-pot process, wherein the confirmation of the target sequence amplification product is obtained by means of a hapten-pair-marked artificial template amplification product. The artificial template sequence is amplified and optionally marked by means of the 5′-cleavage products of the at least one target-sequence-specific FEN probe. The 5′-cleavage product of the at least one target-sequence-specific FEN probe is obtained only if the FEN probe hybridizes, by means of the target-sequence-specific 3′ sequence thereof, to a complementary sequence segment of the at least one target sequence. The detection of the obtained plurality of template amplification products occurs distinctly and preferably by means of immunochromatographic methods.
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