A sample container, sampling system and operating methods permit representative sampling from a liquid phase or boiling liquid, a gaseous phase, a containment sump, containment atmosphere, or condensation chamber of a nuclear power plant following a severe accident. A sample container obtaining an environmental sample includes an outer chamber surrounded by an outer container wall, being directly fluidically connected to the environment through a passage opening in the outer container wall and being fillable with a liquid at least in a base region. An inner chamber surrounded by an inner container wall is fluidically connected to the base region through a passage opening in the inner container wall, has connections for sampling and conveyor medium lines and is otherwise pressure and media tightly sealed from the environment. A pneumatically or hydraulically actuatable closure device for the passage opening between the outer and inner chambers has an actuation medium line connection.