An image reading apparatus includes: a conveying roller; a conveyance guide configured to guide an original to a conveyance path, the conveyance guide including a hole in which the conveying roller is disposed; a first image reading portion configured to read an image on a first side of the original; a second image reading portion configured to read an image on a second side of the original, the second image reading portion disposed below the conveyance guide; a transparent member provided on a position apart from the second image reading portion; and a dustproof member configured to trap a foreign substance falling through a gap between the conveyance guide and the conveying roller at the hole, wherein the dustproof member partitions a space between the conveyance guide and the second image reading portion.