A system and method for synchronizing caliper measurements in a multi-frame 2D image and an anatomical M-mode image is provided. The method may include selecting a frame of a multi-frame 2D image of a region of interest. The method may include positioning a first caliper measurement on the selected frame. The method may include generating an anatomical M-mode image based on a direction of the first caliper measurement. The method may include automatically overlaying a second caliper measurement on the anatomical M-mode image, the second caliper measurement corresponding with the first caliper measurement on the selected frame. The method may include presenting the selected frame having the first caliper measurement simultaneously with the anatomical motion mode image having the second caliper measurement at a display system.