A system for removal of a tissue specimen from an internal body cavity includes a cannula arrangement having an access cannula including a first elongated tubular member, a deployment cannula including a second elongated tubular member configured for insertion into the first lumen, a deployment plunger including a proximal base and a plurality of engagement arms extending distally from the proximal base, and an inner cannula including a third elongated tubular member. The system further includes a containment bag selectively deployable from the cannula arrangement. Distal advancement of the deployment cannula relative to the access cannula deploys a first portion of the containment bag from the cannula arrangement, and distal advancement of the deployment plunger relative to the deployment cannula and the access cannula deploys a second portion of the containment bag from the cannula arrangement.