An interference region setting apparatus capable of setting an interference region in a coordinate system of a mobile robot, with an inexpensive configuration and a little effort. The apparatus has: a shape model storage section configured to store a shape, a position, and an orientation of an obstruction present in a work region of the mobile robot as an obstruction shape model, in a reference coordinate system; a position and orientation calculation section configured to analyze an image, captured by the image capturing apparatus, of a shape feature in a fixed position within the work region, and calculate a position and orientation of the reference coordinate system represented in a robot coordinate system; and an interference region setting section configured to set an interference region based on the position and orientation of the reference coordinate system converted into the robot coordinate system and the stored obstruction shape model.