Invention Grant
- Patent Title: Compositions, methods and systems for polymerase chain reaction assays
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Application No.: US15818601Application Date: 2017-11-20
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Publication No.: US10676778B2Publication Date: 2020-06-09
- Inventor: Adam Lowe , Geoff McDermott , Christopher M. Hindson , Erin R. Chia , Amy L. Hiddessen , Benjamin J. Hindson , Chunxiao Han , Yaqi Wang
- Applicant: Bio-Rad Laboratories, Inc.
- Applicant Address: US CA Hercules
- Assignee: Bio-Rad Laboratories, Inc.
- Current Assignee: Bio-Rad Laboratories, Inc.
- Current Assignee Address: US CA Hercules
- Agency: Kolisch Hartwell, P.C.
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12Q1/6804 ; B01L7/00 ; C12Q1/6844 ; C12Q1/6851 ; B01L3/00
Abstract:
Methods, devices, systems and compositions for detecting nucleic acids in polymerase chain reaction assays, such as droplet digital polymerase chain reaction (ddPCR) assays, using intercalating dyes. A dual surfactant system with at least one fluorosurfactant and at least one non-ionic non-fluorosurfactant may be employed for droplet generation and nucleic acid detection.
Public/Granted literature
- US20180135100A1 COMPOSITIONS, METHODS AND SYSTEMS FOR POLYMERASE CHAIN REACTION ASSAYS Public/Granted day:2018-05-17
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