In one aspect, a system of active noise cancellation includes a signal processing apparatus in operative communication with at least one input device and at least one output device disposed within or proximate to a cabin of a work vehicle. Generally, the signal processing apparatus can be configured to perform a method of active noise cancellation. The method can include receiving an audio input signal from the at least one input device, the audio input signal comprising audio perceptible from within the cabin. The method can also include processing the audio input signal to separate ambient noise in the audio input signal from equipment noise in the audio input signal and generating an output waveform based on the ambient noise, and outputting the output waveform through the at least one audio output device. The output waveform can be configured to at least partially cancel out the ambient noise.