Three-dimensional imaging of a breast tissue is achieved by acquiring a series of X-ray projection images from various angles. A three-dimensional image constructed from the projection images by a computer provides information to the operator to select a coordinate point within the breast from which to obtain a biopsy sample. Acquisition of projection images and construction of three-dimensional images is continued during the insertion of the needle into the breast, during positioning the needle towards the coordinate point, during the sampling and after the sampling. The advantages of utilizing tomosynthesis for breast tissue imaging over stereo imaging include better image quality, easier coordinate measurements within a tissue, ease of use of the equipment by the medical personnel, and fewer image artifact problems.