A microfabricated platform for mimicking the liver zonation and an evaluating method of zone-specific hepatotoxicity using the same is provided. The microfabricated platform for mimicking the liver zonation prepared according to the method of the present invention is divided into three zones of zone 1, zone 2 and zone 3 similarly to in vivo liver tissue and thus the zone-specific hepatotoxicity of a drug in the liver can be evaluated using the same. According to the present invention, the zone-specific hepatotoxicity results can be analyzed quantitatively by using image analysis, so that the platform of the present invention can be effectively used for in vitro screening of zone-specific hepatotoxicity.