Invention Grant
- Patent Title: Expression construct for sensing cell density and substrate availability and its use in conversion of hydroxycinnamic acids
-
Application No.: US14409227Application Date: 2013-06-12
-
Publication No.: US10683521B2Publication Date: 2020-06-16
- Inventor: Matthew Wook Chang , Tat Ming Samuel Lo , Chueh Loo Poh
- Applicant: Nanyang Technological University
- Applicant Address: SG Singapore
- Assignee: Nanyang Technological University
- Current Assignee: Nanyang Technological University
- Current Assignee Address: SG Singapore
- Agency: Seed IP Law Group LLP
- International Application: PCT/SG2013/000242 WO 20130612
- International Announcement: WO2013/191651 WO 20131227
- Main IPC: C12N15/74
- IPC: C12N15/74 ; C12P7/62 ; C12N9/00 ; C12N15/67 ; C12N9/88 ; A61K38/00

Abstract:
An Expression system; isolated nucleic acid molecule or host cell comprising: (i) A first gene encoding for a first enzyme linked to a first promoter, wherein the first promoter is a time delay promoter; (ii) A second gene encoding for a second enzyme capable of using the product generated by the first enzyme as a substrate, wherein the second gene is operably linked to a second promoter, wherein the second promoter is inducible by the product generated by the first enzyme; (iii) Optionally, a third gene encoding a transcription factor that represses expression of the second gene in the absence of the product generated by the first enzyme, wherein the third gene is operably linked to a third promoter that regulates expression of the third gene; and its use in producing a product such as hydroxybenzaldehyde.
Public/Granted literature
Information query
IPC分类: