Methods and apparatuses are provided for transmitting and receiving a signal using a sequence in a wireless communication system. The method includes receiving, from a base station, information indicating whether group hopping or sequence hopping for the reference signal is applied or not; transmitting, to the base station, the signal using a first sequence based on a plurality of sequence groups if the group hopping is applied; and transmitting, to the base station, the signal using a second sequence to which the sequence hopping is applied if the sequence hopping is applied and a number of resource blocks allocated for the second sequence is greater than or equal to a predetermined value.