Embodiments of the invention are directed to a system, method, or computer program product for individual application flow isotope tagging within a network infrastructure. In this regard, the invention is configured to construct a robotic process automation application structured to determine data flow associated with a first technology application within a myriad of data transmission flows between a plurality of network nodes of the network infrastructure. The invention configures a robotic process automation application for inserting a unique isotope tag string in data packets associated with first technology activity performed by the first technology application, prior to transmission from a source network node. The invention is configured to track, in real time, the current locations of the data packets among the multitude of data packets being transmitted in the network, based on the first unique isotope tag string.