An improved method for peak detection in a two-dimensional image is disclosed. In one implementation, the method includes one or more of the following steps: generating a smooth image from the two-dimensional image, detecting a plurality of local peaks in the smooth image, detecting a plurality of true peaks among the plurality of local peaks, and generating a peak-detected image from the smooth image. The smooth image includes a plurality of pixels, where each pixel of the plurality of pixels has an intensity level and an address. The address includes a row number and a column number. The peak-detected image includes a first true peaks subset from the plurality of true peaks. In one implementation, the intensity level of each true peak of the first true peaks subset is higher than an intensity threshold. The method further includes localizing at least one true peak of the first true peaks subset in the peak-detected image.