A light-emitting device and a manufacturing method thereof, and an electronic apparatus are disclosed. The light-emitting device includes: a base substrate, a light-emitting unit, an encapsulation cover plate and a light-guiding structure. The light-emitting unit is on the base substrate; the encapsulation cover plate is opposite to the base substrate and covers the light-emitting unit; and the light-guiding structure is at a light exiting side of the light-emitting unit and includes a plurality of first structures. The light-guiding structure is configured to enable light emitted by the light-emitting unit to be incident into the first structures and enable the light to exit from the encapsulation cover plate.