The present disclosure relates to a pixel driving compensation circuit. The pixel driving compensation circuit can detect and compensate a driving current of a sub-pixel in a pixel unit. The pixel unit includes first, second, and third sub-pixels and the first to third sub-pixels respectively include first, second, and third driving transistors. The pixel driving compensation circuit includes a first switching sub-circuit configured to be turned on in a first period to transmit a driving current output from the first driving transistor to a first detection line, second switching sub-circuit configured to be turned on in a second period to transmit a driving current output from the second driving transistor to a first detection line, and a third switching sub-circuit configured to be turned on in the first period to transmit a driving current output from the third driving transistor to a second detection line.