An air duct sterilization system, and a device and method for producing the air duct sterilization system. The system includes a plurality of ultraviolet light-emitting diodes (UV LEDs), arranged in a UV LED strip within an air duct, configured to destroy airborne biological contaminants in the air duct. The device includes wheel assemblies and an applicator assembly. During installation of the UV LED strip, the wheel assemblies contact a lower surface and an upper surface of an interior of the air duct, thereby enabling the device to roll along the interior of the air duct. The UV LED strip is applied to the upper surface of the interior of the air duct using the applicator assembly, with minimal effort needed from an individual performing the installation. After installation, the UV LEDs are illuminated as needed to destroy airborne biological contaminants, thereby sterilizing the air duct and air therein.