Invention Grant
- Patent Title: Method and kit for DNA typing of HLA gene
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Application No.: US15088727Application Date: 2016-04-01
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Publication No.: US10704095B2Publication Date: 2020-07-07
- Inventor: Takashi Shiina , Shingo Suzuki , Yuki Wada , Shigeki Mitsunaga , Hidetoshi Inoko
- Applicant: GENODIVE PHARMA INC.
- Applicant Address: JP Kanagawa
- Assignee: GENODIVE PHARMA INC.
- Current Assignee: GENODIVE PHARMA INC.
- Current Assignee Address: JP Kanagawa
- Agency: Pearne & Gordon LLP
- Priority: com.zzzhc.datahub.patent.etl.us.BibliographicData$PriorityClaim@120633aa
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12Q1/6881 ; C12Q1/6888

Abstract:
The purpose of the present invention is to provide a method and kit for highly precise DNA typing, in which ambiguity derived from phase ambiguity is eliminated. The present invention provides a method for the DNA typing of HLA, which is characterized by comprising: (1) a step of preparing a set of primers which can respectively anneal specifically to an upstream region and a downstream region of each of HLA-A, HLA-B, HLA-C, HLA-DQA1, HLA-DQB1, HLA-DPA1 and HLA-DPB1 gene in the nucleotide sequence for the human genome, and a set of primers which can respectively anneal specifically to exon-2 and a 3′-side non-translated region in HLA-DRB1; (2) a step of carrying out the PCR amplification of a sample to be tested (DNA) using the sets of primers; (3) a step of determining the nucleotide sequence for a PCR-amplified product; and (4) an optional step of carrying out the homology search in a data base.
Public/Granted literature
- US20160208326A1 Method and Kit for DNA Typing of HLA Gene Public/Granted day:2016-07-21
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