An apparatus for the collection of multiple samples of a fluid stream. The apparatus includes a plurality fluid collection containers arranged to receive separate portions of the fluid stream in a temporal sequence and a sealing mechanism provided on each fluid collection container configured to close the fluid collection containers when filled by a portion of the fluid stream and to cause the fluid stream to flow to the next fluid collection container in the sequence. The fluid collection containers separately collect at least a beginning portion of the fluid stream, a mid-portion of the fluid stream and an end-portion of the fluid stream. Each of the fluid collection containers may be detachably connected to tubing held in a housing having a sloped upper surface for receiving the fluid stream and an inlet for directing the fluid stream to the tubing. The housing may be a fluid collection cup.