A machinery for preparation and analysis of one or more slides for a biological material according to an immuno-fluorescence technique includes a loading station for one or more containers of test liquids and/or samples of product; a positioning station of the slides; a containment station of one or more slide covers; an analysis station having a microscope that acquires digital images at one or more magnifications; an assembly, mobile inside the area defined by the machinery and controlled to move through the stations to prepare one or more slides according to a predefined protocol and arrange them in a corresponding analysis station for subsequent acquisition of digital images; one or more covers for each slide arranged in the seat; a container of the covers, the assembly being controlled to remove the covers once the phase of pre-dilution of the samples has been completed and arrange them inside the container.