A diffusion cell is described that includes a receiver compartment and a tightening ring intended to be positioned above the receiver compartment in such a way that a membrane sample, such as a sample of a skin, can be arranged between the receiver compartment and the tightening ring. The cell further includes means for tightening by rotation of the tightening ring on the receiver compartment. Uses of such a diffusion cell, in particular for evaluating the penetration capacity of a compound of interest contained in an aerosol formulation, are also described. These uses can be applicable in an occlusive patch or semi-occlusive patch or in a formulation under occlusive or semi-occlusive conditions in the skin. The uses can also be implemented to evaluate the capacity of an aerosol formulation, an occlusive or semi-occlusive patch or a formulation under occlusive or semi-occlusive conditions to deliver a compound of interest through the skin.