A vehicle detection system and method is disclosed. The vehicle detection system can comprise an imaging device arranged on a vehicle and at least one light source that is configured to illuminate a target area arranged beneath the vehicle to excite at least one reactive agent contained within a fluid sample disposed at the target area. A detection device is coupled to the imaging device and configured to detect an intensity of light reflected from the target area and generate an output signal indicative of a color characteristic of the at least one reactive agent. An electronic data processor is communicatively coupled to the imaging device, light source, and detection device, and is configured to associate the detected color characteristic of the at least one reactive agent with a fluid system of the vehicle, and display a location of the fluid system on an operator interface.