The present disclosure provides an organic light-emitting display panel and a manufacturing method thereof. The organic light-emitting display panel may include: a first anode layer including a number of first anodes; a pixel definition layer defining a number of pixel regions, wherein one of a first opening and a second opening is defined in each of the pixel regions, the second opening has a depth smaller than that of first opening, an inclination angle is formed between the side wall and the bottom surface, and the inclination angle is larger than 90° and smaller than 180°; a second anode layer arranged in second opening; an organic light-emitting layer arranged in the first opening and the second opening. Accordingly, the resolution of the organic light-emitting display panel is improved.