Disclosed are a Multiple-Input Multiple-Output (MIMO) system-based signal detection method. The method includes: performing a scaling calculation on a first covariance matrix according to first main diagonal elements in the first covariance matrix to obtain a second covariance matrix; obtaining a whitening matrix according to the second covariance matrix; taking the whitening matrix, a vector of a receiving signal and a channel matrix as input parameters, and inputting the parameters into a mathematical model for a whitening operation and perform a whitening calculation to obtain an operation result; and detecting a transmit signal in a MIMO system according to the operation result to obtain a detection result. Also disclosed are a MIMO system-based signal detection device and a computer storage medium.