Transducer assemblies that can be used in acoustophoretic systems are disclosed. The acoustophoretic systems including the transducer assemblies and methods of operating the acoustophoretic systems are also disclosed. The transducer assemblies include a housing, a polymeric film attached to the housing, and a piezoelectric material attached to the polymeric film. The piezoelectric material is not attached to, and does not come in direct contact with, the housing. The piezoelectric material is configured to be driven by a drive signal to create a multi-dimensional acoustic standing wave. The piezoelectric material can be attached to the polymer film by an adhesive coating on the polymer film.