Invention Grant
- Patent Title: Method and kit for multiplex DNA typing of HLA gene
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Application No.: US14786690Application Date: 2014-05-09
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Publication No.: US10711306B2Publication Date: 2020-07-14
- Inventor: Takashi Shiina , Shingo Suzuki , Yuki Wada , Shigeki Mitsunaga , Hidetoshi Inoko
- Applicant: Genodive Pharma Inc.
- Applicant Address: JP Kanagawa
- Assignee: GENODIVE PHARMA INC.
- Current Assignee: GENODIVE PHARMA INC.
- Current Assignee Address: JP Kanagawa
- Agency: Pearne & Gordon LLP
- Priority: com.zzzhc.datahub.patent.etl.us.BibliographicData$PriorityClaim@61efd815
- International Application: PCT/JP2014/062433 WO 20140509
- International Announcement: WO2014/181854 WO 20141113
- Main IPC: C12P19/34
- IPC: C12P19/34 ; C12Q1/6881

Abstract:
The purpose of the present invention is to provide a method and kit for highly precise DNA typing, in which a high throughput sequencer is used and ambiguity derived from phase ambiguity is eliminated. The present invention provides a method for the DNA typing of HLA, which is characterized by comprising: (1) a step of preparing sets of primers which respectively hybridize specifically to an upstream region and a downstream region of at least 2 genes selected from genes belonging to HLA class I and HLA class II in a human genome sequence, and are capable of amplifying under the same PCR conditions; (2) a step of simultaneously amplifying said at least 2 genes in a test sample (DNA) using the sets of primers in a single container under the same PCR conditions; (3) a step of determining the nucleotide sequences of PCR amplified products; and (4) a step of optionally carrying out a homology search within a database.
Public/Granted literature
- US20160060695A1 METHOD AND KIT FOR MULTIPLEX DNA TYPING OF HLA GENE Public/Granted day:2016-03-03
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