The present invention provides a diesel power generation system using biofuel capable of reliably generating electric power using biofuel so as not to coagulate, whereby biofuel is used to drive the diesel engine and the generator is driven based on the driving force of the diesel engine to generate electricity, while driving the diesel engine with the desired efficiency, the biofuel is not solidified without deteriorating at high temperature.The diesel power generation system 10 using the biofuel of the present invention is provided with a first fuel heater 22 for heating the biofuel sent by the fuel feed pump 20 through the fuel feed circuit 18 to a second temperature range set lower than the first temperature range in which the diesel engine 14 is driven with a predetermined efficiency, a second fuel heater 24 disposed in a portion of the fuel feed circuit 18 between the first fuel heater 22 and the diesel engine 14 and heating the biofuel flowing therethrough, a temperature sensor 26 interposed on the upstream side of the diesel engine 14 of the fuel feed circuit 18 and measuring the temperature of biofuel flowing into the diesel engine 14, and, a control device 28 for controlling the second fuel heater 24 so that the biofuel flowing into the diesel engine 14 is maintained within the first temperature range, based on the measurement result of the temperature sensor 26.