Provided is a separator that is used in a nonaqueous electrolyte secondary battery, and that exhibits high impregnation ability with respect to a nonaqueous electrolyte solution. The separator for a nonaqueous electrolyte secondary battery disclosed herein is provided with: a porous substrate; a first coat layer formed on a pair of main surfaces and on an inner surface of the porous substrate; and a second coat layer formed on the first coat layer, at least on one main surface of the porous substrate. The first coat layer has higher hydrophilicity than the porous substrate. The second coat layer has higher hydrophobicity than the porous substrate. The hydrophilicity of an inner surface of the separator for a nonaqueous electrolyte secondary battery is higher than the hydrophilicity of the second coat layer, in a central region of the separator for a nonaqueous electrolyte secondary battery in the thickness direction thereof.