An apparatus is disclosed for separating and preserving biomolecules of a biological fluid sample. The apparatus includes an assembly having sides forming a hollow shape having a first opening at one end and second opening at the opposite end, a sample mixing chamber positioned adjacent the first opening within the assembly, the sample mixing chamber from which a flow of the biological fluid sample is actuated in a direction from the sample mixing chamber to the first matrix layer, the sample mixing chamber being in a direction downstream of the first opening, a first valve positioned between the sample mixing chamber and the first matrix layer, the first valve configured to control the flow to the first matrix layer, a first input in fluid communication with the sample mixing chamber and positioned upstream of the first valve, a second input positioned between the first matrix layer and the second matrix layer, and a second valve positioned between the second matrix layer and the second opening, the second valve configured to control the flow to the second matrix layer.