Fastener alignment tools and methods of utilizing the same are disclosed herein. The fastener alignment tools are configured to operatively engage a plurality of fasteners with a corresponding plurality of fastening locations and include a gripping region and an alignment tool body. The alignment tool body defines a plurality of fastener-receiving regions. Each fastener-receiving region includes a fastener receptacle, which is configured to receive a corresponding fastener in a received orientation relative to the alignment tool body, and a fastener retention structure, which is configured to retain the corresponding fastener within the fastener-receiving region at a retained orientation relative to the alignment tool body. The methods include positioning each fastener of the plurality of fasteners within a corresponding fastener-receiving region, retaining each fastener within the corresponding fastener-receiving region, orienting the fastener alignment tool relative to the plurality of fastening locations, and simultaneously releasing each fastener.