Disclosed is a shut-off valve installed in an accommodation chamber regulating entry/exit of air. The shut-off valve includes: a body removably disposed in the accommodation chamber and having multiple air passage holes; a valve stem adapted to be moved vertically along a valve stem insertion hole formed in the body; a disc mounted on a disc engagement portion formed at a lower end of the valve stem to open/close at least one of the multiple air passage holes; a rotor disposed on an upper side of the body to vertically move the valve stem, the rotor being provided with an opening/closing indicator to check opening/closing of the shut-off valve outside the accommodation chamber, wherein the accommodation chamber has an upper wall, a lower wall, and a side wall, the body disposed passing through the upper wall and lower wall of the accommodation chamber, and the disc is located outside the accommodation chamber.