Disclosed embodiments concern histochemical process compositions comprising at least one nanoparticle in an amount effective to reduce or substantially eliminate the average number of spots per slide that result from a sample staining protocol. The nanoparticle can be any of various nanoparticles, or combinations thereof, including metals, metal alloys, metal oxides, ceramics, functionalized metals or metalloids, and other miscellaneous nanoparticles, such as carbon nanoparticles and diamond nanoparticles. Typically, the nanoparticle concentration is from greater than zero to at least about 25 parts per million, more typically from about 2 parts per million to about 20 parts per million. Embodiments of a method for using process nanosolutions also are disclosed. One embodiment concerns applying a histochemical process composition to a sample, followed by performing a staining protocol on the sample. Particular embodiments concern automated histochemical processes comprising dispensing a nanosolution onto a sample using an automated system, heating the sample, and performing a sample staining process on the sample.