There is provided a light source device including a first light source that emits light in a first wavelength region, a second light source that emits light in a second wavelength region different from the first wavelength region, a wavelength conversion unit including a fluorescent material and emits fluorescent emission light in a different wavelength region upon irradiation with the light in the first wavelength region, and a combining unit that has wavelength selectivity to a specific wavelength region corresponding to the second wavelength region and combines the light in the first wavelength region from the first light source, the light in the second wavelength region from the second light source, and the fluorescent emission light which are incident on the combining unit with one another.