A system including: at least one processor; and at least one memory having stored thereon instructions that, when executed by the at least one processor, control the at least one processor to: receive image data of a sequence of images, and a current image of the sequence of images being after a previous image in the sequence of images, each of the current and previous images including a cell; filter the current image to remove noise; iteratively deconvolve the filtered current image to identify edges of the cell within the current image based on determined edges of the cell within the previous image; and segment the deconvolved current image to determine edges of the cell within the current image.