This disclosure provides an intravascular memory metal puncture needle and the use thereof. The puncture needle comprises a split-shaped structure capable of opening and closing, as well as a metal spiral hollow tube including a plurality of spiral coils, wherein the split-shaped structure and the metal spiral tube are made of memory metal, and the split-shaped structure is composed of a plurality of tapered petals; when the temperature is T1, the pitch between two adjacent spiral coils in the metal spiral tube is decreased, each of tapered petals in the split-shaped structure is closed; when the temperature is T0, the pitch between two adjacent spiral coils in the metal spiral tube is increased, and each of tapered petals is opened. The puncture needle achieves synergistic effect by matching the metal spiral tube with the split-shaped structure, so that it not only move well in the blood vessels, but also accurately pierce the blood vessel wall, and achieve effective sampling for biopsy.