Provided is a color filter substrate, including a plurality of pixel unit regions arranged in an array. Each of the pixel unit regions has a first sub-pixel region, a second sub-pixel region, and a third sub-pixel region, which are respectively provided thereon with a red color barrier, a green color barrier, and a blue color barrier. In at least one of the pixel unit regions, thickness of a color barrier provided on a sub-pixel region corresponding to color of a specific color barrier is larger than thickness of a color barrier provided on a sub-pixel region corresponding to color of each of other two color barriers.