Provided is a tissue slicing device having a first motor, a filling assembly, a filter, a pipe, and a cutter. The filling assembly includes a tube and a piston rod movably inserted through the tube. One end of the piston rod abuts against the first motor. The filter is located at an end of the tube opposite the first motor. The pipe is located at a common side of the first motor and the filling assembly, and includes a nozzle located at an end of the pipe and proximal to an end of the filter opposite the filling assembly. The cutter is proximal to the filter. The guiding body is located at the end of the tube opposite the first motor. Provided is an apparatus having the tissue slicing device, such that manpower and time can be saved, and the tissue slicing degree can be unified.