The present disclosure relates to systems and methods directed to alignment of light-emitting devices. An example method includes coupling a support substrate to a light guide assembly. The support substrate includes a plurality of elongate structures and a plurality of laser diodes. Each laser diode of the plurality of laser diodes is coupled to a respective elongate structure of the plurality of elongate structures. The method also includes causing a given laser diode of the plurality of laser diodes to emit light toward the light guide assembly. The light guide assembly includes a light guide manifold configured to guide at least a portion of the emitted light as transmitted light. The method additionally includes comparing at least one optical characteristic of the transmitted light to a desired optical characteristic and, based on the comparison, adjusting a position of the given laser diode by adjusting the respective elongate structure.