A display substrate includes a base substrate; a black matrix on the base substrate and in the black matrix region, the black matrix comprising a plurality of rows and a plurality of columns intersecting each other; a first color filter layer having a net structure with a plurality of openings, and comprising a first portion in the first region and a second portion in the black matrix region, the first color filter layer substantially covering the plurality of columns of the black matrix; a second color filter layer in the second region; and a third color filter layer in the third region. A first height of a surface of the first portion away from the base substrate relative to the base substrate and a second height of a surface of the second portion away from the base substrate relative to the base substrate are within 30% of each other.