A display substrate (100) is provided for a display device. The display substrate (100) includes a display region with an alignment film formed on one side of the display region. A non-display region at a peripheral area of the substrate and adjacent to the display region. An uneven layer (1) formed on the non-display region at a same side as the alignment film. A first film layer (2) formed on the uneven layer (1) and covering the uneven layer (1) to form a substantially flat surface of the non-display region with respect to the display region.