A fixture for eccentric clamping of a wheel is composed of a base (1), a cylinder (2), a cylinder support (3), a connecting seat (4), a movable positioning seat (5), a guide rail sliding seat (6), a linear guide rail (7) and a fixed positioning seat (8), wherein the cylinder support (3), the linear guide rail (7) and the fixed positioning seat (8) are fixed on the base (1), the cylinder (2) is mounted on the cylinder support (3), an output shaft of the cylinder (2) is connected with the connecting seat (4), the connecting seat (4) is connected with the linear guide rail (7) via the guide rail sliding seat (6), the movable positioning seat (5) is fixed on the connecting seat (4), and the cylinder (2) is configured to drive the connecting seat (4) and the movable positioning seat (5) to move along the linear guide rail (7).