Provided are a method, a device and a system for manipulating presentation content of screen transmission. The method includes: receiving play start information for presentation content at a screen transmission source end transmitted by a corresponding screen transmission hardware, and starting a preset play manipulation mode at a screen transmission presentation end (S11); detecting a preset touch operation signal in the play manipulation mode, and obtaining a preset play control instruction corresponding to the touch operation signal (S12); and controlling, according to the play control instruction, the presentation content at the screen transmission presentation end to enter a corresponding play state (S13). Based on this, it is possible to implement manipulation of the presentation content on a large touch screen, facilitate users' operations, and advantageously improve the user experience.