Systems and methods for automated sequencing database generation are disclosed herein. The system can include memory that can include a content library database; a graph database; and a model database. The system can include a user device and at least one server. The at least one server can: receive a content aggregation from the content library database; identify content components of the content aggregation based on a natural language processing analysis of at least a portion of the content aggregation; identify explicit sequencing of the content components; generate an intermediate content graph based on the explicit sequencing of the content components; generate a final content graph from the intermediate content graph based on implicit sequencing of the content components; and store the final content graph within the graph database.