The present disclosure discloses a pixel circuit and a driving method thereof, a display substrate and a display device, the pixel circuit includes: an initialization sub-circuit, a writing compensation sub-circuit, a light-emitting enabling sub-circuit, a light-emitting device, and a driving sub-circuit, the light-emitting enabling sub-circuit is configured to couple the driving sub-circuit to the light-emitting device under control of an enabling signal terminal. The initialization sub-circuit is configured to input a voltage of an initial voltage terminal to a gate of a driving transistor in the driving sub-circuit under control of a reset signal terminal to initialize the driving sub-circuit. The writing compensation sub-circuit is configured to input, under control of a scanning signal terminal, a data voltage output from a data signal terminal to the driving sub-circuit to perform data compensation on the driving sub-circuit.