An image pickup apparatus including: an image pickup device including a pixel region in which image pixels corresponding to microlenses are arrayed in a matrix, each of the image pixels being divided into a plurality of focus detection pixels configured to generate respective photoelectric conversion signals; and a control circuit configured to control a cycle in a column direction of first rows in which photoelectric conversion signals in an image pixel are added up to generate an image pixel signal, and second rows in which both of a pair of the focus detection pixel signals or one of the pair of the focus detection pixel signals and the image pixel signal are generated and read, in a frame is provided.