Invention Grant
- Patent Title: Modified template-independent enzymes for polydeoxynucleotide synthesis
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Application No.: US16113757Application Date: 2018-08-27
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Publication No.: US10760063B2Publication Date: 2020-09-01
- Inventor: J. William Efcavitch , Julie L. Tubbs , Prem Sinha , Boguslaw Stec
- Applicant: Molecular Assemblies, Inc.
- Applicant Address: US CA San Diego
- Assignee: Molecular Assemblies, Inc.
- Current Assignee: Molecular Assemblies, Inc.
- Current Assignee Address: US CA San Diego
- Agency: Brown Rudnick LLP
- Agent Thomas C. Meyers
- Main IPC: C12N9/12
- IPC: C12N9/12 ; C12P19/34
Abstract:
The invention includes methods for identifying polymerases, such as modified terminal nucleotidyl transferases (TdT), that are capable of binding nucleotides comprising removable 3′-O-blocking moieties to a nucleic acid initiator, without the use of a template. The invention further includes the identified polymerases, and methods of using the polymerases for de novo synthesis of predetermined oligonucleotide sequences.
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