A printing method for biochip fabrication is implemented by a printing apparatus including a platform for supporting a substrate, a pipet module, and a control module. The method includes: moving the platform in sequence to multiple positions associated with multiple to-be-printed points on the platform; moving the pipet module reciprocatively toward and away from the platform during each movement of the platform; discharging, by the pipet module, solution sample onto the respective one of the to-be-printed points to form a bio-sensing spot to form a biochip; and determining, by the control module, whether to execute a supplementary printing procedure for the biochip based on whether each bio-sensing spot satisfies a predetermined criterion.