A method of producing a sugar liquid includes a step of filtering a saccharified liquid derived from cellulose-containing biomass, through a nanofiltration membrane and/or reverse osmosis membrane; and a two-step washing step of washing the nanofiltration membrane and/or reverse osmosis membrane after the filtration, with an acid washing liquid and then with an alkali washing liquid; is provided. The method of producing a sugar liquid in which a cellulose-derived sugar liquid is processed through a nanofiltration membrane and/or reverse osmosis membrane is/are effectively washed in a contaminated separation membrane(s).