A disposable test kit comprising a base sheet; a sealing sheet having at least one sealed sample collection opening before use of the test kit; at least one test strip sealed between the base sheet and the sealing sheet before use of the test kit; and at least one top sheet layered over the sealing sheet before use of the test kit to keep the at least one sample collection opening sealed, the at least one top sheet further configured to be at least partially detached from the sealing sheet to open the at least one sample collection opening and expose a sample collection portion of the at least one test strip for receiving a fluid analyte thereon during use of the test kit.