Invention Grant
- Patent Title: Compositions and methods for improved cell-based botulinum neurotoxin assays
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Application No.: US16246201Application Date: 2019-01-11
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Publication No.: US10768178B2Publication Date: 2020-09-08
- Inventor: Ward C. Tucker , Timothy M. Piazza
- Applicant: BIOMADISON, INC.
- Applicant Address: US CA Del Mar
- Assignee: BioMadison, Inc.
- Current Assignee: BioMadison, Inc.
- Current Assignee Address: US CA Del Mar
- Agency: Fish IP Law, LLP
- Main IPC: G01N33/569
- IPC: G01N33/569 ; C12Q1/37 ; G01N21/64 ; C12Q1/6897 ; A47C15/00
Abstract:
Methods for a cell-based assay for Botulinum neurotoxin are provided in which a transfected cell that produces a reporting peptide is contacted with a Botulinum neurotoxin in media having a sub-physiological osmolarity and a temperature that is above physiological temperature. This combination provides an unexpected synergistic effect in reducing the EC50 of the cell-based assay relative to an analogous cell-based assay performed at physiological osmolarity and temperature.
Public/Granted literature
- US20190145974A1 COMPOSITIONS AND METHODS FOR IMPROVED CELL-BASED BOTULINUM NEUROTOXIN ASSAYS Public/Granted day:2019-05-16
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